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Effect of Ulva lactuca on Indole-3-acetic acid, Siderophores and Phosphatase Production by Azospirillum brasilense NH Growing in Seawater as Natural Medium |
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PP: 35-38 |
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Author(s) |
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El Hafid Nabti,
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Abstract |
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| In this paper we aim to synthesize a new culturemedium similar to the seawater, and test the osmotolerant strain Azospirillum
brasilense NH for IAA and siderophores-production and phosphate solubilization in presence of a natural osmoprotectant Ulva lactuca
(marine alga). This is in order to reveal the capacity of this strain to restore a plant growth irrigated with seawater or brackish water in
the future. Azospirillum brasilense NH a new isolate from Algerian soil known as an osmotolerant bacterium was tested with natural
medium containing seawater (32g/l NaCl) in presence of ethanol algal extracts and absence of other carbon source such as malic
acid, for growth and indole acetic acid production. The results obtained showed that bacterium NH increased it growth at 25% of
algal extracts and produced high concentration of auxin phytohormone indole acetic acid from tryptophan (64.61μg/ml) in absence of
NH4Cl. Siderophores production and inorganic phosphates solubization were tested then positive results were obtained.These results
demonstrated that A.brasilense NH get it natural environment in seawater in presence of algal extracts. So, the capability of the strain
to keep the Plant Growth Promoting Rhizobacteria characters under seawater using U.lactuca to save it growth, let us think about an
eventual application as a biofertilizer in agricultural soils irrigated with saline water or menaced with salinity. As we demonstrated
already, that this strain stimulated a wheat growth under saline conditions, but, here, we have to look for an application of the bacterium
in large scale. Because, now we need to save the crop invaded by seawater and other saline waters. |
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