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Pathway of 1,2,4-Trichlorobenzene Degradation by Bacillus mucilaginosus Isolated from Petroleum Polluted Soils |
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PP: 49-59 |
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doi:10.18576/jehe/040201
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Author(s) |
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M.A. M. Abo-State,
Y. E. Saleh,
O. A. A. Khalil,
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Abstract |
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| An indigenous bacterial strain MAM-24 was isolated from petroleum polluted soil by selective enrichment with 1,2,4-trichlorobenzene (1,2,4-TCB) as the sole source of carbon and energy. During growth on 1,2,4-TCB, growth by measuring O.D., protein measurement and bacterial count were determined. A stoichiometric amount of chloride ions is released. The degradation percentage of 1,2,4-TCB was quantified by high performance liquid chromatography (HPLC). Bacillus mucilaginosus MAM-24 which have been identified by 16 S rRNA in a previous study was the best 1,2,4-TCB degrader. It could degrade 99.3%, 98.0%, 98.6%, 99.3% and 14.4% of 5, 10, 15, 25, 50 μM 1,2,4-TCB respectively. Degradation products of 1,2,4-TCB was identified by gas chromatography mass spectrometry (GC-MS) analysis which revealed that isolate MAM-24 dechlorinated 1,2,4-TCB as a first step. The corner stone of the intermediates was acetophenone. |
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