Login New user?  
International Journal of Nanomaterials and Chemistry
An International Journal
               
 
 
 
 
 
 
 
 
 
 
 
 

Content
 

Volumes > Volume 1 > No. 2

 
   

Biosynthesis of Copper Nanoparticles Using Aqueous Phyllanthus Embilica (Gooseberry) Extract- Characterisation and Study of Antimicrobial Effects

PP: 53-63
Author(s)
G. Caroling, E. Vinodhini, A. Mercy Ranjitham, P. Shanthi.,
Abstract
Vegetable mediated synthesis of nanoparticles is a green chemistry approach that connects nanotechnology and biotechnology. In the present investigation we have used a fast, convenient and environment friendly method for the synthesis of copper nanoparticles by biologically reducing CuSO4 with aqueous extract of Goose Berry (Phyllanthus Embilica) under optimum conditions (pH-6-10). The formation of copper nanoparticles was indicated by the colour change from colourless to brown. Biosynthesized nanoparticles were characterized by UV-VIS, FT-IR, XRD, SEM, and EDAX analysis. These biologically synthesised Cu nanoparticles were tested for antimicrobial activity against four human pathogens viz. Staphylococcus Aureus and Escheria Coli. The reduction process was simple and convenient to handle and monitored by UV-Vis spectroscopy which showed surface plasmon resonance at 294 nm. The presence of active proteins and phenolic groups present in biomass before and after reduction was identified by FT-IR. The crystalline morphology and size of the nanoparticles were determined by SEM and X-ray diffraction studies, which showed the average particle size of Cu nanoparticles was in the range 15-30nm as well as revealed their FCC structure. Presence of elemental copper was revealed by EDAX analysis. These biologically synthesised Cu nanoparticles were found to be effective in controlling growth of human pathogens viz. Staphylococcus Aureus and Escheria Coli. The reducing property of aqueous extract is due to the presence of antioxidant viz. ascorbic acid, polyphenols which is confirmed by quantitative assay.

  Home   About us   News   Journals   Conferences Contact us Copyright naturalspublishing.com. All Rights Reserved